| A Home for Everybunny | Coralville | B | $26K |
| A Purposeful Life Animal Rescue & S | Marion | C | $34K |
| Aheinz57 Pet Rescue & Transport Inc | De Soto | C | $904K |
| All Creatures Considered Foundation | Carroll | F | $233K |
| Almost Home Animal Rescue | Chariton | B | $135K |
| Animal Lifeline of Iowa Inc | Des Moines | C | $726K |
| Animal Rescue League of Iowa Inc | Des Moines | A | $12.99M |
| Animal Rescue League of Marshalltown | Marshalltown | C | $598K |
| Animal Rescue of Carroll Inc | Carroll | C | $170K |
| Animal Resource Foundation of Iowa | Center Point | C | $7,364 |
| Animal Welfare Friends | Monticello | C | $590K |
| Animal Welfare Services | Boone | B | $215K |
| Ape Cognition & Communication Institute | Des Moines | D | $509K |
| Bailing Out Benji | Ames | C | $483K |
| Betsy's Barn | Cylinder | F | $18K |
| Bite Size Vegan | Iowa City | C | $69K |
| Blank Park Endowment Corporation | Des Moines | D | $422K |
| Blank Park Zoo Foundation Inc | Des Moines | B | $11.91M |
| Bonobo Hope Initiative Inc | Waukee | F | $29K |
| Boone Area Humane Society Inc | Boone | B | $495K |
| Buchanan County Animal Shelter Inc | Hazleton | F | $243K |
| Caring Paws Rescue | Storm Lake | F | $523K |
| Cedar Bend Humane Society | Waterloo | C | $1.35M |
| Cedar Creek Sanctuary Inc | Fonda | F | $143K |
| Cedar Rapids Animeals | Cedar Rapids | D | $3,229 |
| Cedar Valley Humane Society | Cedar Rapids | C | $2.36M |
| Clinton Humane Society | Clinton | C | $562K |
| Council Bluffs Kennel Club | Council BLFS | C | $36K |
| Country Sunsets & Kitties Sanctuary | Murray | D | $18K |
| Cows Foundation | Fairfield | B | $72K |
| Cresco Wildlife Club | Cresco | A | $114K |
| Crisis Canines of the Midlands | Reasnor | A | $172K |
| Critter Crusaders of Cedar Rapids Inc | Cedar Rapids | C | $495K |
| Cyclone Navdha Inc | Story City | C | $8,331 |
| Des Moines County Humane Society | Burlington | D | $425K |
| Destiny Youth Ranch Inc | Alvord | C | $262K |
| Dogs Forever of Iowa | Cedar Rapids | C | $196K |
| Dubuque Regional Humane Society | Dubuque | B | $1.68M |
| East Texas Hoof and Paw Animal Rescue | Dysart | F | $130K |
| Emmet County Animal Shelter Inc | Estherville | D | $283K |
| Flat Coated Retriever Foundation | Burlington | D | $20K |
| Floyd County Humane Society Inc | Charles City | D | $205K |
| Fluffybutts Rescue Resort Inc | Washington | C | $58K |
| Forever Home Dog Rescue | Indianola | A | $68K |
| Foundation for the Boone Area | Boone | D | $55K |
| Freedom Run Dog Park Corp | New Hampton | D | $4,670 |
| Friends Helping Friends Foundation of Cedar Rapids | Cedar Rapids | D | $14K |
| Friends of Boyer Chute and Desoto National Wildlife Refuge | Missouri Vly | D | $27K |
| Friends of Neal Smith National Wildlife | Prairie City | D | $201K |
| Friends of the Animal Center Foundation | Iowa City | C | $587K |
| Friends of the Animal Shelter Inc | Sioux City | C | $110K |
| Friends of the Animals | Tipton | D | $154K |
| Friends of the Mines of Spain | Dubuque | D | $32K |
| Fur Fun Rescue | Lisbon | C | $335K |
| Furever Friends Rescue of Appanoose County | Centerville | C | $230K |
| Furry Friends Refuge | WDM | D | $524K |
| Giving Back Outdoors | WDM | C | $560K |
| Glee Fielding Trust Under Will | Sioux City | D | $58K |
| Great Outdoors Foundation | WDM | C | $5.59M |
| Greenbelt Humane Society | Iowa Falls | D | $90K |
| Hamilton County Animal Advocates | Stratford | C | $275K |
| Happy Doggo | Ames | C | $4.15M |
| Harrison County Humane Society | Logan | D | $232K |
| Heart Animal Rescue | Decorah | B | $88K |
| Heartland Humane Society LTD | Ottumwa | C | $861K |
| Heros Haven Animal Rescue | Coralville | B | $179K |
| Hooves & Paws Rescue of the Heartland | Glenwood | F | $187K |
| Hope After Racing Thoroughbreds | Altoona | C | $251K |
| Hope Animal Rescue | WDM | C | $94K |
| Humane Society of North Central Iow | Fort Dodge | D | $490K |
| Humane Society of North Iowa | Mason City | D | $1.33M |
| Humane Society of Northeast Iowa | Decorah | D | $334K |
| Humane Society of Northwest Iowa | Milford | B | $394K |
| Humane Society of Scott County | Davenport | B | $2.76M |
| Humboldt County Animal Shelter Inc | Humboldt | C | $223K |
| Iowa Bird Rehabilitation | Des Moines | C | $264K |
| Iowa Farm Sanctuary | Oxford | C | $897K |
| Iowa Great Lakes Fishing Club | Spencer | B | $87K |
| Iowa Humane Alliance | Cedar Rapids | B | $818K |
| Iowa Parrot Rescue | Letts | C | $106K |
| Iowa Wildlife Center | Ames | F | $324K |
| It Takes a Village Animal Rescue | Muscatine | F | $625K |
| Jackson County Humane Society | Maquoketa | C | $173K |
| K 9 Kindness Rescue Inc | Bettendorf | C | $167K |
| K9Sensus Foundation | Lucas | D | $609K |
| Keokuk Humane Society | Keokuk | D | $21K |
| Kiya Koda Humane Society | Indianola | C | $771K |
| Last Hope Animal Rescue Inc | Cedar Rapids | C | $392K |
| Lee County K-9 Association | Montrose | D | $9,001 |
| Lucky Bulldog Rescue | Glenwood | B | $84K |
| Marion County Humane Society | Knoxville | B | $377K |
| Marshalltown Feral Support Inc | Marshalltown | B | $83K |
| Mid-Iowa Retriever Club Inc | Colfax | A | $51K |
| Midlands Humane Society | Council BLFS | D | $1.31M |
| Midwest Pets for Life | Clinton | A | $66K |
| Monarch Research Project | Marion | C | $1.33M |
| Muddy Paws Second Chance Rescue | Council BLFS | C | $326K |
| Muscatine Humane Society Inc | Muscatine | D | $180K |
| Muscatine Humane Society Inc | Muscatine | D | $598K |
| Niabi Zoological Society | Eldridge | D | $63K |
| Noahs Ark Foundation Inc | Fairfield | C | $244K |
| Noahs Hope Animal Rescue Inc | Sioux City | D | $439K |
| Oh My Dog Rescue | Madrid | B | $194K |
| Our Turn Inc | Dubuque | C | $22K |
| Palomino Horse Breeders Heritage Found Inc | Greenfield | F | $37K |
| Panora Pets | Panora | B | $23K |
| Paws-N-Claws Iowa | Newton | D | $122K |
| People for Paws | Shenandoah | C | $57K |
| People for Pets | Spencer | C | $331K |
| Pet Project Midwest | Des Moines | A | $114K |
| Porter-Rinsky Foundation | Norwalk | C | $19K |
| Poweshiek Animal League Shelter Humane Society | Grinnell | C | $203K |
| Promise 4 Paws Dog Rescue Inc | Council BLFS | D | $86K |
| Protectors of Animal Welfare Inc | Fort Madison | F | $615K |
| Raccoon River Pet Rescue | Perry | C | $198K |
| Raptor Resource Project | Decorah | B | $464K |
| S O L a S Support Our Local Animal Shelter | Council BLFS | B | $86K |
| Safe Haven of Iowa County | Williamsburg | C | $305K |
| Safe Paws | Boone | C | $126K |
| Saving Animals in Need Today | Cedar Rapids | C | $133K |
| Savings Our Avian Resources Soar | Dedham | B | $92K |
| Sharon's Project Foundation Inc | Anamosa | C | $395 |
| Short Noses and Friends United Resc | Council BLFS | C | $202K |
| Siouxland Humane Society Inc | Sioux City | C | $1.55M |
| Stephen Animal Shelter Foundation | Oskaloosa | F | $25K |
| Stephen Memorial Animal Shelter Inc | Oskaloosa | C | $322K |
| Tama County Humane Society | Toledo | C | $92K |
| Three Labs Foundation | Carroll | D | $107K |
| Transboundary Animal Biologics Inc | Ames | A | $72K |
| Wet-Nose Rescue Inc | Lemars | C | $216K |
| Whiskers TNR of Warren County | Martensdale | B | $107K |
| Whispurring Hope Rescue | Dubuque | D | $50K |
| Wildlife Lodge & Clinic | Sioux City | D | $97K |
| Wildthunder Wildlife Bat Reptile Raptor & Animal Rehabilitation and | Independence | C | $94K |